Binding Assay:Article Title: Antibodies specific for epidermal growth factor receptor variant III and their uses
Article Snippet: .. To assess the cell binding specificity of anti-EGFRvIII antibodies generated from the AlivaMab mice, three isogenic rat glioblastoma cell lines and a human cancer cell line were used: F98 (does not express any form of human EGFR), F98-EGFRwt (expresses wild-type EGFR), F98-npEGFRvIII (expresses EGFRvIII) and A431 (an epidermoid carcinoma cell line with wild-type EGFR over-expression), all obtained from American Type Culture Collection (Manassas, Va.). .. For cell staining, 500,000 cells were incubated with 50 μl hybridoma supernatants for 45 min at 4° C., washed with binding buffer (PBS (Phosphate Buffered Saline)+0.5% BSA (Bovine Serum Albumin)), followed by incubation with FITC-conjugated goat anti-mouse Fc specific secondary antibody from Jackson ImmunoResearch Laboratories (West Grove, Pa.).
Article Title: Chimeric antigen receptors targeting epidermal growth factor receptor variant III
Article Snippet: .. To assess the cell binding specificity of anti-EGFRvIII antibodies generated from the AlivaMab mice, three isogenic rat glioblastoma cell lines and a human cancer cell line were used: F98 (does not express any form of human EGFR), F98-EGFRwt (expresses wild-type EGFR), F98-npEGFRvIII (expresses EGFRvIII) and A431 (an epidermoid carcinoma cell line with wild-type EGFR over-expression), all obtained from American Type Culture Collection (Manassas, Va.). .. For cell staining, 500,000 cells were incubated with 50 μl hybridoma supernatants for 45 min at 4° C., washed with binding buffer (PBS (Phosphate Buffered Saline)+0.5% BSA (Bovine Serum Albumin)), followed by incubation with FITC-conjugated goat anti-mouse Fc specific secondary antibody from Jackson ImmunoResearch Laboratories (West Grove, Pa.).
Article Title: Antibodies specific for epidermal growth factor receptor variant III and their uses
Article Snippet: .. To assess the cell binding specificity of anti-EGFRvIII antibodies generated from the AlivaMab mice, three isogenic rat glioblastoma cell lines and a human cancer cell line were used: F98 (does not express any form of human EGFR), F98-EGFRwt (expresses wild-type EGFR), F98-npEGFRvIII (expresses EGFRvIII) and A431 (an epidermoid carcinoma cell line with wild-type EGFR over-expression), all obtained from American Type Culture Collection (Manassas, Va.). .. For cell staining, 500,000 cells were incubated with 50 μl hybridoma supernatants for 45 min at 4° C., washed with binding buffer (PBS (Phosphate Buffered Saline)+0.5% BSA (Bovine Serum Albumin)), followed by incubation with FITC-conjugated goat anti-mouse Fc specific secondary antibody from Jackson ImmunoResearch Laboratories (West Grove, Pa.).
Article Title: Chimeric antigen receptors targeting epidermal growth factor receptor variant III
Article Snippet: .. To assess the cell binding specificity of anti-EGFRvIII antibodies generated from the AlivaMab mice, three isogenic rat glioblastoma cell lines and a human cancer cell line were used: F98 (does not express any form of human EGFR), F98-EGFRwt (expresses wild-type EGFR), F98-npEGFRvII (expresses EGFRvIII) and A431 (an epidermoid carcinoma cell line with wild-type EGFR over-expression), all obtained from American Type Culture Collection (Manassas, Va.). .. For cell staining, 500,000 cells were incubated with 50 μl hybridoma supernatants for 45 min at 4° C., washed with binding buffer (PBS (Phosphate Buffered Saline)+0.5% BSA (Bovine Serum Albumin)), followed by incubation with FITC-conjugated goat anti-mouse Fc specific secondary antibody from Jackson ImmunoResearch Laboratories (West Grove, Pa.).
Generated:Article Title: Antibodies specific for epidermal growth factor receptor variant III and their uses
Article Snippet: .. To assess the cell binding specificity of anti-EGFRvIII antibodies generated from the AlivaMab mice, three isogenic rat glioblastoma cell lines and a human cancer cell line were used: F98 (does not express any form of human EGFR), F98-EGFRwt (expresses wild-type EGFR), F98-npEGFRvIII (expresses EGFRvIII) and A431 (an epidermoid carcinoma cell line with wild-type EGFR over-expression), all obtained from American Type Culture Collection (Manassas, Va.). .. For cell staining, 500,000 cells were incubated with 50 μl hybridoma supernatants for 45 min at 4° C., washed with binding buffer (PBS (Phosphate Buffered Saline)+0.5% BSA (Bovine Serum Albumin)), followed by incubation with FITC-conjugated goat anti-mouse Fc specific secondary antibody from Jackson ImmunoResearch Laboratories (West Grove, Pa.).
Article Title: Chimeric antigen receptors targeting epidermal growth factor receptor variant III
Article Snippet: .. To assess the cell binding specificity of anti-EGFRvIII antibodies generated from the AlivaMab mice, three isogenic rat glioblastoma cell lines and a human cancer cell line were used: F98 (does not express any form of human EGFR), F98-EGFRwt (expresses wild-type EGFR), F98-npEGFRvIII (expresses EGFRvIII) and A431 (an epidermoid carcinoma cell line with wild-type EGFR over-expression), all obtained from American Type Culture Collection (Manassas, Va.). .. For cell staining, 500,000 cells were incubated with 50 μl hybridoma supernatants for 45 min at 4° C., washed with binding buffer (PBS (Phosphate Buffered Saline)+0.5% BSA (Bovine Serum Albumin)), followed by incubation with FITC-conjugated goat anti-mouse Fc specific secondary antibody from Jackson ImmunoResearch Laboratories (West Grove, Pa.).
Article Title: Antibodies specific for epidermal growth factor receptor variant III and their uses
Article Snippet: .. To assess the cell binding specificity of anti-EGFRvIII antibodies generated from the AlivaMab mice, three isogenic rat glioblastoma cell lines and a human cancer cell line were used: F98 (does not express any form of human EGFR), F98-EGFRwt (expresses wild-type EGFR), F98-npEGFRvIII (expresses EGFRvIII) and A431 (an epidermoid carcinoma cell line with wild-type EGFR over-expression), all obtained from American Type Culture Collection (Manassas, Va.). .. For cell staining, 500,000 cells were incubated with 50 μl hybridoma supernatants for 45 min at 4° C., washed with binding buffer (PBS (Phosphate Buffered Saline)+0.5% BSA (Bovine Serum Albumin)), followed by incubation with FITC-conjugated goat anti-mouse Fc specific secondary antibody from Jackson ImmunoResearch Laboratories (West Grove, Pa.).
Article Title: Chimeric antigen receptors targeting epidermal growth factor receptor variant III
Article Snippet: .. To assess the cell binding specificity of anti-EGFRvIII antibodies generated from the AlivaMab mice, three isogenic rat glioblastoma cell lines and a human cancer cell line were used: F98 (does not express any form of human EGFR), F98-EGFRwt (expresses wild-type EGFR), F98-npEGFRvII (expresses EGFRvIII) and A431 (an epidermoid carcinoma cell line with wild-type EGFR over-expression), all obtained from American Type Culture Collection (Manassas, Va.). .. For cell staining, 500,000 cells were incubated with 50 μl hybridoma supernatants for 45 min at 4° C., washed with binding buffer (PBS (Phosphate Buffered Saline)+0.5% BSA (Bovine Serum Albumin)), followed by incubation with FITC-conjugated goat anti-mouse Fc specific secondary antibody from Jackson ImmunoResearch Laboratories (West Grove, Pa.).
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